Summer Research Program for Science Teachers
South Shore High School, Brooklyn
August 2003
How do we genera
Objectives:
£
Students should understand the
concept of PCR (Polymerase Chain Reaction).
o
Standard C (Life Science)
o
Standard B (Physical
Science)
£
Students should understand the
applications of DNA testing.
o
Standard E (Science and
Technology)
o
Standard F (Science in
Personal and Social Perspectives)
£
Students should be prepared for
their hands on experience in the labs that follow this lesson.
o
Standard A (Science as
Inquiry)
£
Students
should become interested in using the internet as a learning resource.
o
Standard E (Science and
Technology)
£
Students should become interested
in presenting information with PowerPoint.
o
Unifying Concepts and
Processes
Materials:
£
Computer with Microsoft Powerpoint installed and connected to the internet
£
(A class set of computers)
£
A projector
£
A white screen
Motivation:
Sometimes we need to know,
if a certain gene is present – for example to identify the carrier of a
genetic disease. The DNA that can
be obtained from a sample – be it a human tissue sample or a bacterial plasmid
– is usually not enough to determine the presence or absence of a gene beyond
doubt. That is why a certain
technique called PCR (Polymerase Chain Reaction) is used to create larger
amounts of the gene of interest.
Information:
Virtual Lab:
can
be used as a demonstration or, if enough computers are available, as an
interactive experience for the students.
CONNECT
TO THE INTERNET. GO TO http://hhmi.org/biointeractive/vlabs/index.htm
CLICK
ON Bacterial ID Lab
READ
introduction
CLICK
to enter the lab
READ
the instructions and
FOLLOW
the procedure for step 1 (Sample Prep)
and step 2 (PCR Amplification).
Summary
(Homework):